91麻豆精品一二三区在线,精品无码久久久久成人漫画,久久蜜桃精品av人妻,中国老熟妇自拍hd发布

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>動(dòng)物巨噬細(xì)胞>CRL-28433D4/21 豬肺泡巨噬細(xì)胞

3D4/21 豬肺泡巨噬細(xì)胞

簡(jiǎn)要描述:CRL-2843 3D4/21 豬肺泡巨噬細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-2843
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問  量:4207

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

ATCC 細(xì)胞|細(xì)胞株|細(xì)胞系|腫瘤細(xì)胞|巨噬細(xì)胞

3D4/21 (ATCC® CRL-2843™)

Organism Sus scrofa, pig

Tissue  lung

Cell Type  macrophage macrophage (alveolar); immortalized with SV40 large T antigen transformed with pSV3-neo

Product Format  frozen

Morphology  macrophage

Culture Properties  adherent

Biosafety Level  2  [Cells contain SV40 viral DNA sequences]

Age  27 days

Gender  unknown

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Strain  Landrace

Applications

These porcine myelomonocytic cell lines may have a wide variety of applications in porcine virology and immunology Ref.



Storage Conditions  liquid nitrogen vapor phase

Derivation  The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid.


Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Virus Susceptibility  Bovine adenovirus 3

Classical swine fever virus , Classical swine fever virus

Human parainfluenza virus 3

Swinepox virus

Vesicular stomatitis New Jersey virus

Porcine adenovirus

Herpes simplex virus 1

African swine fever virus

Pseudorabies virus

Vaccinia virus

CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Swine vesicular disease virus

Comments

The plasmid carries the genes for neomycin resistance and SV40 large T antigen.


A subpopulation of each cell line (3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844)) was positive, to varying degrees depending on the media formulation, for nonspecific esterase activity and phagocytosis.


Clone 3D4/21 can produce Bovine adenovirus type 3 (BAV-3) to markedly higher titers than clones 3D4/2 and 3D4/31.


Addition of DMSO improved the capability of clone 3D4/21 to replicate the field isolate of African swine fever virus (ASFV/Lillie) compared to the other clones.

Complete Growth Medium  RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate supplemented with 0.1 mM nonessential amino acids, 90%; fetal bovine serum, 10%

Subculturing

Volumes used in this protocol are for 75cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 5 x 103 to 7 x 103 viable cells/cm2 is recommended.

Incubate cultures at 37°C. Subculture when cell concentration reaches between 3 x 105 and 4 x 105 cells/cm2.

Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended

Medium Renewal: Two to three times weekly

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.


CRL-2843   3D4/21  豬肺泡巨噬細(xì)胞

Cryopreservation

Complete growth medium supplemented with 5% (v

) DMSO. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X.

Culture Conditions

Temperature: 37°C

Atmosphere: 5% CO2 in air recommended























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
国产精品99久久精品爆乳| 自拍偷自拍亚洲精品10p| 欧美40老熟妇色xxxxx| 岚皋县| 欧美激情| 乐亭县| 娱乐| 精品人妻少妇嫩草av| 日本一区二区三区视频在线观看| 91丨porny丨丰满熟女| а√天堂8资源中文在线| 精品国产免费久久久久久婷婷| 亚洲精品主播一区二区三区| 人人妻人人澡人人人爽人人dvd| 中文国产成人精品久久APP| 99精品无人区乱码1区2区3区| 亚洲va久久久噜噜噜久久天堂| 狠狠色综合激情丁香五月| h无码精品动漫在线观看| 天天做天天爱夜夜爽| 久久精品女人的天堂av| 野花高清在线观看免费官网| 精品乱子伦一区二区三区| 久久99精品久久久大学生| 两个女人互添下身视频在线观看 | 精品卡一卡二卡乱码高清| 影院午夜精品久久久久久99热| 亚洲精品国产品国语在线app| 久久黄色福利视频中文字幕| 色一情一乱一伦| 国产欧美日本aⅴ精品| 日韩制服国产精品一区| 久久99久久久久婷婷| 中文字幕乱码人妻综合二区三区 | 欧美色aⅴ欧美综合色| 国产最新进精品视频| 免费男人下部进女人下部视频| 精产国品一二三产品麻豆| 久久精品超碰av无码| 久久青草国产免费频观| 伦理在线播放|