91麻豆精品一二三区在线,精品无码久久久久成人漫画,久久蜜桃精品av人妻,中国老熟妇自拍hd发布

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>小鼠正常細(xì)胞>CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

簡要描述:CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞,
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|;
細(xì)胞庫管理規(guī)范,
提供的細(xì)胞株背景清楚,
提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號:
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時間:2025-03-27
  • 訪  問  量:2102

產(chǎn)品分類

Product Category

詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞 的詳細(xì)介紹

CRL-1715 TM4 正常小鼠睪丸Leydig細(xì)胞

ATCC® Number:CRL-1715™  Price:$329.00
Designations:TM4

Depositors:JP Mather

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:epithelial


Source:Organ: testis
Disease: normal
Cell Type: Sertoli cell;


Cellular Products:retinol binding protein

tissue plasminogen activator

transferrin



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:follicle stimulating hormone (FSH), expressed

androgen receptor, expressed

progesterone receptor, expressed



Tumorigenic:No

Antigen Expression:H-Y antigen; Mus musculus, expressed

Age:11 to 13 days

Gender:male

Comments:The TM4 cell line is reported to respond to FSH with an increase in cAMP production, but to not respond to luteinizing hormone (LH). The FSH responsiveness is much reduced compared to primary sertoli cell cultures. Constitutive plasminogen activator production is reported to be low, but is stimulated by FSH and, to a greater extent, by retinoic acid.

Tested and found negative for ectromelia virus (mousepox).



Propagation:ATCC complete growth medium: A 1:1 mixture of Ham'S F12 medium and Dulbecco's modified Eagle's medium with 1.2 g/L sodium bicarbonate and 15 mM HEPES, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell supension to new culture vessels.

  6. Incubate cultures at 37C.


Subc*tion Ratio: A subc*tion ratio of 1:10 to 1:50 is recommended
Medium Renewal: Every 3 to 4 days


Preservation:Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040



References:1158: Mather JP. Establishment and characterization of two distinct mouse testicular epithelial cell lines. Biol. Reprod. 23: 243-252, 1980. PubMed: 6774781

1159: Mather JP, et al. Culture of testicular cells in hormone-supplemented serum-free medium. Ann. N.Y. Acad. Sci. 383: 44-68, 1982. PubMed: 7046561

1184: Carson DD, et al. Synthesis and secretion of a novel binding protein for retinol by a cell line derived from Sertoli cells. J. Biol. Chem. 259: 3117-3123, 1984. PubMed: 6538197

26150: Mather JP, Phillips DM. Establishment of a peritubular myoid-like cell line and interactions between established testicular cell lines in culture. J. Ultrastruct. Res. 87: 263-274, 1984. PubMed: 6544874



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗證碼:

    請輸入計算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號:滬ICP備10013034號-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
夜色88v精品国产亚洲av小说| 美女AV一区二区三区| 射进来av| 综合成人| 国产中文区3幕区2021| 奇米第四色一二三四区| 国产精品视频一区二区三区无码 | 人妻一本久道久久综合久久鬼色| 无码人妻一区二区三区免费看成人| 灵台县| 久久精品人人做人人爱爱漫画| 四虎影视在线播放| 熟女乱论网| 天堂在线v| 色性av| 日韩麻豆| 中文人妻熟妇乱又伦精品| 国产乱国产乱| 五十路六十路熟女| 久久黄色影院| 午夜亚洲精品| 草草影院第一页yycccom| 久草av在线播放| 少妇高潮喷水视频| 欧美精品av| 欧美v片| 孕交视频| 阿v天堂在线| 少妇一级淫片免费看…| 中国熟妇xxx| 被4个男人摁着强进了好爽 | 熟女少妇精品一区二区| 99精品国产再热久久无毒不卡| 天天天天做夜夜夜夜做无码| 精品无码久久久久成人漫画 | 中文日本字幕MV在现线观看| 精品久久BBBBB免| 内射人妻无码色AV| 国久久| 加勒比在线| 伊人狠狠操|