91麻豆精品一二三区在线,精品无码久久久久成人漫画,久久蜜桃精品av人妻,中国老熟妇自拍hd发布

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>豬腎細(xì)胞系>CCL-33 PK-15 豬腎細(xì)胞系

CCL-33 PK-15 豬腎細(xì)胞系

簡(jiǎn)要描述:CCL-33 PK-15 豬腎細(xì)胞系,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-14
  • 訪  問(wèn)  量:4049

產(chǎn)品分類

Product Category

詳細(xì)介紹

CCL-33 PK-15 豬腎細(xì)胞系,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件

CCL-33 PK-15 豬腎細(xì)胞系 的詳細(xì)介紹


ATCC® Number:CCL-33™  Price:$355.00
Designations:PK(15)

Depositors:Cutter Laboratories, Inc.

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Sus scrofa (pig)

Morphology:epithelial

Source:Organ: kidney
Disease: normal


Cellular Products:plasminogen activator; keratin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (Roche FuGENE® Transfection Reagents)

Virus Resistance:poliovirus 2

Age:adult

Comments:The presence of a porcine papovavirus in PK(15) cells has been reported in cells obtained from multiple sources including the ATCC . [53398] [53399]

The Foreign Animal Disease Diagnostic Laboratory of the US Department of Agriculture has determined that ATCC CCL-33 is not infected with Hog cholera virus or African swine fever virus, and uses this line to screen for those viruses. [105588]

The cell line harbors an endogenous C-type retrovirus. [26185] [53399] [56104]

The cells are positive for porcine circovirus (PCV) antigens.

The cells are positive for keratin by immunoperoxidase staining.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturing:Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week

Rinse the cell sheet 2 times with fresh 0.25% trypsin, 0.03% EDTA solution, remove trypsin and allow the culture to stand at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.



Preservation:culture medium 95%; DMSO, 5%

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020



References:22988: Dulac GC, Afshar A. Porcine circovirus antigens in PK-15 cell line (ATCC CCL-33) and evidence of antibodies to circovirus in Canadian pigs. Can. J. Vet. Res. 53: 431-433, 1989. PubMed: 2686830

26184: Pirtle EC, Woods LK. Cytogenetic alterations in swine kidney cells persistently infected with hog cholera virus and propagated with and without antiserum in the medium. Am. J. Vet. Res. 29: 153-164, 1968. PubMed: 4965860

26185: Armstrong JA, et al. C-type virus particles in pig kidney cell lines. J. Gen. Virol. 10: 195-198, 1971. PubMed: 4324256

53398: Newman JT, Smith KO. Characteristics of a swine papovavirus. Infect. Immun. 5: 961-967, 1972. PubMed: 4344097

53399: Tumilowicz JJ, et al. Concurrent replication of a papovavirus and a C-type virus in the CCL 33 porcine cell line. In Vitro 15: 922-928, 1979. PubMed: 232060

56104: Todaro GJ, et al. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74, 1974. PubMed: 4132403

105588: James A House, personal communication



















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
亚洲蜜桃av| 亚洲中文字幕无码天然素人| 久久精品国产亚洲av四区| 韩国激情三小时未删减片段| 欧美黄色小说| 飘零影院电视剧免费观看| 国产精品入口66mio女同| 亚洲AV日韩综合一区尤物| 亚洲国产精品无码久久九九大片健 | 亚洲精品国精品久久99热| av在线精品| 色一情一乱一乱一区免费网站| av高清在线| 欧美成人精品一区二区男人小说 | 超碰操| 真人做受120分钟免费看| 夜色阁亚洲一区二区三区| 一边做饭一边躁狂怎么办| 午夜福利电影无码专区| 亚洲日韩欧美视频二区国产 | 亚洲 中文 欧美 日韩 在线| 石柱| 无棣县| 久久这里只有精品首页| 天天躁久久躁日日躁| 久久久久亚洲精品无码网址色欲| 色综合久久成人综合网| 边做饭边被躁欧美三级| 久久国产情侣露脸精品| 免费A级毛片无码A∨免费| 天海翼一区二区三区高清在线 | 国产精品久久久久无码AV色戒| 97视频精品全国免费观看| 野花视频在线观看免费| 美女脱个精光露出尿口视频| 欧美a一级| 国产亚洲精品久久久麻豆男与男| 亚洲a在线观看| 99精品免费久久久久久久久日本| 沟厕白嫩大屁股国产盗摄| 旺苍县|